Efficacy

EFFICACY DATA for
mPerial DETERGENT/DISINFECTANT
(EPA Reg. No. 1839-79-83129)

 

 

 

VIRUCIDAL DATA:

Test Methods:
* U.S. E.P.A. Pesticide Assessment Guidelines, Subdivision G: Product Performance, 1982, Section 91-30,
pp. 72-76.
† Virucide Assay (EPA, Federal Register 10, No. 123, 6/25/75, p. 26836)
▪ Protocols for Testing the Efficacy of Disinfectants against Hepatitis B Virus (HBV) (EPA, Federal Register,
Vol., 65, No. 166, 8/25/2000, p. 51828).
‡ Protocol for Testing Disinfectants against Hepatitis C Virus using Bovine Viral Diarrhea Virus as approved
by the U.S. EPA on August 15, 2002.
Test Conditions: 2 oz/gal dilution, 10 minute contact time, tested in the presence of serum glass petri dish
substrates

Results:

Test Organism Sample Titer Reduction
†Adenovirus Type 2 A 3.0 log10
A >3.0 log10
‡Bovine Viral Diarrhea Virus (BVDV) A 6.1 log10
A 3.8 log10
*Feline Calicivirus (FCV) A 5.79 log10
B >6.06 log10
▪Hepatitis B Virus (HBV) (Duck Hepatitis B Virus-DHBV) A 4.5 log10
B >4.5 log10
‡Hepatitis C Virus (HCV) (Bovine Viral Diarrhea Virus-BVDV) A 6.1 log10
B >3.8 log10
†Herpes Simplex Type 1 (Sabin) A >4.0 log10
B >3.7 log10
*Human Coronavirus (ATCC VR-740, strain 229E) A >3.0 log10
B >3.0 log10
*Human Immunodeficiency Virus, HTLV-IIIRF, strain of HIV-1 (associated withAIDS) A >3.0 log10
B >3.0 log10
†Influenza A2(Japan 305/57) RF, strain of HIV-1 (associated withAIDS) A >6.5 log10
B >6.0 log10
*Norovirus (Norwalk Virus) (FCV) A 5.79 log10
B >6.06
log10
†Vaccinia (Wyeth) A >3.5 log10
B >3.5 log10

Conclusion: Under the conditions of this investigation, mPerial
Detergent/Disinfectant was virucidal for Adeno-virus Type 2, Bovine Viral
Diarrhea Virus (BVDV), Feline Calicivirus (FCV), Hepatitis B Virus (HBV),
Hepatitis C Virus (HCV), Herpes Simplex Type 1 (Sabin), Human Coronavirus,
Human Immunodeficiency Virus (HIV-1), Influenza A2
(Japan 305/57), Norovirus
(Norwalk Virus) and Vaccinia (Wyeth) according to criteria established by the U.S.
Environmental Protection Agency for registration and labeling of a disinfectant
product as a virucide.

 

 

 

 

MILDEW FUNGISTATIC DATA:
Test Method: Hard Surface Mildew Fungistatic Test (Unofficial Protocol, 10/27/76)
Test Organism: Aspergillus niger (ATCC 6275)
Test Conditions: tile substrates

Results:

Sample Dilution No. of Exposed Tiles No. of Tiles Showing Growth
NP 4.5 2 oz/gal 10 0
Control 10 10

Conclusion:

Under the conditions of this investigation, mPerial Detergent/Disinfectant was fungistatic for Aspergillus
niger according to criteria established by the U. S. Environmental Protection Agency for registration and
labeling of a disinfectant product as a fungistat.

FUNGICIDAL DATA:

Test Method: AOAC Fungicidal Test
Test Organism: Trichophyton mentagrophytes (ATCC 9533)
Test Conditions: 2 oz/gal dilution
5% organic soil load
20°C exposure temperature

Results:

PHENOL RESISTANCE
Exposure Time
(min.) vs. Growth
Exposure Time
(min.) vs. Growth
Sample 5 10 15 Dilution 5 10 15
A + 0 0 1:60 + 0 0
B + 0 0 1:70 + + +
1:60 + 0 0
1:70 + + 0
+ = growth      0 = no growth

Conclusion:

Under the conditions of this investigation, mPerial Detergent/Disinfectant was fungicidal for Trichophyton
mentagrophytes according to criteria established by the U. S. Environmental Protection Agency for
registration and labeling of a disinfectant product as a fungicide.

DISINFECTION DATA:

Test Method: AOAC Use Dilution
Test Conditions: 5% organic soil load, 10 minute contact time, stainless steel carrier substrates,
20°C exposure temperature, 2 oz/gal dilution

Results:

PHENOL RESISTANCE
No. of Carriers(min.) vs. Growth Exposure Time
(min.) vs. Growth
Test Organism Sample Exposed Positive Dilution 5 10 15
Staphylococcus aureus
(ATCC 6538)
A 60 0 1:60 + 0 0
B 60 0 1:70 + + +
C 60 0
Salmonella choleraesuis
(ATCC 10708)
A 60 0 1:90 + 0 0
B 60 0 1:100 + + +
C 60 0
Pseudomonas aeruginosa
(ATCC 15442)
A 60 0 1:80 + 0 0
B 60 0 1:90 + + +
C 60 0
Clostridium difficile
(ATCC 9689)
A 10 0 1:110 + 0 0
B 10 0 1:120 + + +
C 60 0
Enterobacter aerogenes
(ATCC 13048)
A 10 0 1:80 + 0 0
B 10 0 1:90 + + +
Escherichia coli
(ATCC 11229)
A 10 0 1:90 + 0 0
B 10 0 1:100 + + +
Klebsiella pneumoniae
(ATCC 4352)
A 10 0 1:60 + 0 0
B 10 0 1:70 + + +
Salmonella schottmuelleri
(ATCC 8759)
A 10 0 1:90 + 0 0
B 10 0 1:100 + + +
Shigella dysenteriae
(ATCC 12180)
A 10 0 1:90 + 0 0
B 10 0 1:100 + + +
Staphylococcus aureus (Methicillin
resistant) (MRSA) (ATCC 33593)
A 10 0 Not determined; published
information not available.
B 10 0
Staphylococcus aureus (Vancomycin
intermediate resistant) (VISA)
A 10 0 Not determined; published
information not available.
B 10 0
Streptococcus faecalis
((ATCC 10541)
A 10 0 1:70 + 0 0
B 10 0 1:80 + + +
Streptococcus pyogenes (Clinical-Flesh
Eating Strain, BIRD M3)
A 10 0 Not determined; published
information not available.
B 10 0
Streptococcus salivarius A 10 0 1:120 + 0 0
(ATCC 9222) B 10 0 1:130 + + +
+ = growth 0 = no growth

Conclusion:

Under the conditions of these investigations, mPerial Detergent/Disinfectant demonstrated disinfectant
activity against Staphylococcus aureus, Salmonella choleraesuis, Pseudomonas aeruginosa,Clostridium
difficile, Brevibacterium ammoniagenes, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae,
Salmonella schottmuelleri, Shigella dysenteriae, Methicillin resistant Staphylococcus aureus (MRSA),
Vancomycin intermediate resistant Staphylococcus aureus (VISA), Streptococcus faecalis, Streptococcus
pyogenes (Clinical – Flesh Eating Strain, BIRD M3) and Streptococcus salivarius according to criteria
established by the U. S. Environmental Protection Agency for registration and labeling of a disinfectant
product as a bactericide.


SANITIZATION DATA:

Test Method: AOAC Germicidal and Detergent Sanitizing Action of Disinfectants
Test Conditions: 200 ppm active quaternary
2 oz/3.5 gal dilution

Results:

TOTAL BACTERIAL COUNTS/
% KILL vs. EXPOSURE TIME
Synthetic
Hard Water
30 seconds 60 seconds
Test Organism Sample (ppm) TBC * % Kill† TBC * % Kill†
Staphylococcus aureus
(ATCC 6538)
A
B
C
250
250
250
1120
1065
1275
99.999
99.999
99.999
65
70
185
99.999
99.999
99.999
Staphylococcus aureus
(ATCC 6538)
A
B
C
250
250
250
1120
1065
1275
99.999
99.999
99.999
65
70
185
99.999
99.999
99.999
* TBC = Total Bacterial Count, cfu/ml  † % Kill calculated based on initial
inoculum control count of 75-125
x 106 cfu/ml.

Conclusion:

Under the conditions of these investigations, mPerial Detergent/Disinfectant demonstrated sanitizing activity
against Staphylococcus aureus and Escherichia coli according to criteria established by the U. S.
Environmental Protection Agency for registration and labeling of a disinfectant product as a sanitizer.